Nov 14, 2019

Postdoctoral position: DNP-enhanced biomolecular solid-state NMR-spectroscopy for the study of protein (mis)folding #DNPNMR

A postdoctoral position is available in the group of Henrike Heise at Forschungszentrum Juelich/Heinrich Heine University Duesseldorf

The successful candidate will study disordered and aggregation-prone proteins in all stages of the misfolding pathway. Further, interaction with potential drug candidates will be elucidated. State-of-the art solid-state NMR-spectroscopy, involvng polarization enhancement by DNP and fast magic angle spinning will be applied to elucidate those processes at atomic resolution. A selection of relevant publication is given below.

Our laboratory at the Forschungszentrum Jülich is equipped with state of the art solid-state NMR spectrometers operating at 800 and 600 MHz, DNP equipment is available for 600 and 800 MHz spectrometers. An ultrafast MAS probe head for spinning speeds up to > 100 kHz will be available soon, the installation of an ultrahigh field spectrometer operating at 1.2 GHz is scheduled for 2020.

The Forschungszentrum Jülich offers a vibrant research environment with close collaborations with the University of Düsseldorf, the RWTH Aachen University and the Max-Planck Institute Mülheim. Our laboratory is part of the Jülich center of structural biology JuStruct which bundles expertise with infrastructure in the field of atomic resolution of structural biology (NMR in solution and the solid state, X-ray crystallography,cryo-EM, molecular modelling and neutron scattering).

The initial appointment is for a year, with a possibility of renewal.The initial appointment is for a year, with a possibility of renewal.

Applications will be reviewed on a rolling basis, and candidates will be considered until the position is filled.

The ideal candidate should have a strong background in biomolecular NMR-spectroscopy. Experience with hyperpolarization and/ or Protein expression and purfication is a plus. Applicants must submit a cover letter summarizing research experience and specifying the interests in this position; a curriculum vitae (including a publication list); a statement of research interests; and two letters of reference to henrike.heise@hhu.de

Information about our group:

Selection of relevant publications:
  • L. Siemons, B. Uluca-Yazgi, R. B. Pritchard, S. McCarthy, H. Heise, D. F. Hansen, Determining isoleucine side-chain rotamer-sampling in proteins from 13C chemical shift, Chem.Commun. 2019, in press. doi:10.1039/C9CC06496F.
  • A. König, D. Schölzel, B. Uluca, T. Viennet, Ü. Akbey, H. Heise, Hyperpolarized MAS NMR of unfolded and misfolded proteins, Solid State NMR, 2019, 98, 1-11.
  • B. Uluca, T. Viennet, D. Petrović, H. Shaykhalishahi, F. Weirich, A. Gönülalan, B. Strodel, M. Etzkorn, W. Hoyer, and H. Heise. DNP-Enhanced solid-state NMR at Cryogenic Temperatures: a Tool to Snapshot Conformational Ensembles of α-Synuclein in Different States. Biophys. J. 2018, 114, 1614-1623.
  • L. Gremer, D. Schölzel, C. Schenk, E. Reinartz, J. Labahn, R. Ravelli, M. Tusche, C. Lopez-Iglesias, W. Hoyer, H. Heise, D. Willbold, G. Schröder, Fibril structure of amyloid-ß(1-42) by cryo-electron microscopy, Science, 2017, 358, 116-119.
  • T. Viennet, A. Viegas, A. Kuepper, S. Arens, V. Gelev, O. Petrov, T. N. Grossmann, H. Heise, M. Etzkorn, Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization. Angew. Chem. Int. Ed. Engl. 2016, 55, 10746-10750.
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Forschungszentrum Juelich GmbH
52425 Juelich
Sitz der Gesellschaft: Juelich
Eingetragen im Handelsregister des Amtsgerichts Dueren Nr. HR B 3498
Vorsitzender des Aufsichtsrats: MinDir Volker Rieke
Geschaeftsfuehrung: Prof. Dr.-Ing. Wolfgang Marquardt (Vorsitzender),
Karsten Beneke (stellv. Vorsitzender), Prof. Dr.-Ing. Harald Bolt,
Prof. Dr. Sebastian M. Schmidt
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