Sep 20, 2019

Design of a local quasi-distributed tuning and matching circuit for dissolution DNP cross polarization #DNPNMR

Vinther, Joachim M.O., Vitaliy Zhurbenko, Mohammed M. Albannay, and Jan Henrik Ardenkjær-Larsen. “Design of a Local Quasi-Distributed Tuning and Matching Circuit for Dissolution DNP Cross Polarization.” Solid State Nuclear Magnetic Resonance 102 (October 2019): 12–20.


Dynamic nuclear polarization (DNP) build-up times at low temperature for low-gamma nuclei can be unfavorably long and can be accelerated by transfer of polarization from protons. The efficiency of the cross polarization (CP) depends on the B1-field strengths, the pulse sequence chosen for cross polarization and the sample composition. CP experiments rely on high B1-fields, which typically lead to electrical discharge and breakdown in the circuit. This problem is particularly severe in the low pressure helium atmosphere due to easily ionized helium atoms. The purpose of this study is to identify strategies to minimize voltages across components in a tuning and matching circuit of the coil to avoid electrical discharge during CP experiments. Design equations for three tuning and matching network configurations are derived. The results of the study are then used in the design of a single coil double resonance DNP probe operating at 71.8 MHz (13C frequency) and 285.5 MHz (1H frequency). In the current setup we achieve 28% polarization on 13C in urea with a build-up time of 11.6 min with CP compared to 14% and 53 min by direct polarization using TEMPOL as the radical. Different cross polarization sequences are compared.

Sep 18, 2019

[NMR] Dnp workshop October 22-24 at NHMFL



The NIH funded P41 "National Resource for Advanced NMR Technology" is sponsoring an in-depth workshop on DNP-enhanced MAS ssNMR at the National High Magnetic Field Lab (NHMFL) NMR facility October 22-24, 2019, in Tallahassee, FL.

The workshop will focus on practical aspects of MAS-DNP NMR, including technical details of instrumentation, maintenance, theory and best practices for ssNMR spectroscopy, and samples preparation strategies for optimal DNP. Significant time will be spent in lab working with the 600 MHz MAS-DNP instrument, a benchtop X-band EPR instrument, preparing samples, and collecting DNP data. The content is designed for graduate students, postdoctoral researchers, and staff scientists who are pursuing MAS-DNP measurements in their studies, and participants are encouraged to bring a trial sample for characterization on the instruments.

Registration is limited to 5 participants, who will be responsible for travel and lodging. Lunch will be provided.

For application details, please contact Amy Howe, amy.howe@ufl.edu . The regional SEMRC conference (https://nationalmaglab.org/news-events/events/for-scientists/south-east-magnetic-resonance-conference) will be held directly after the workshop.

Joanna R. Long, PhD
Professor of Biochemistry & Molecular Biology
Director, Advanced Magnetic Resonance Imaging & Spectroscopy Facility
Assoc. Director, National High Magnetic Field Laboratory
McKnight Brain Institute, LG-187
Box 100245
University of Florida
Gainesville, FL 32610

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Hyperpolarized Water Enhances Two-Dimensional Proton NMR Correlations: A New Approach for Molecular Interactions #DNPNMR

Sadet, Aude, Cristina Stavarache, Mihaela Bacalum, Mihai Radu, Geoffrey Bodenhausen, Dennis Kurzbach, and Paul R. Vasos. “Hyperpolarized Water Enhances Two-Dimensional Proton NMR Correlations: A New Approach for Molecular Interactions.” Journal of the American Chemical Society 141, no. 32 (August 14, 2019): 12448–52.


Proteins and peptides interactions are characterized in the liquid state by multidimensional NMR spectroscopy experiments, which can take hours to record. We show that, starting from hyperpolarized HDO, two-dimensional (2D) proton correlation maps of a peptide, either free in solution or interacting with liposomes, can be acquired in less than 60 s. In standard 2D NMR spectroscopy without hyperpolarization, the acquisition time required for similar spectral correlations is of the order of hours. This hyperpolarized experiment allows to identify amino-acids featuring solvent-interacting hydrogens and provides fast spectroscopic analysis of peptide conformers. These experiments are a useful and straightforward tool for biochemistry and structural biology, as they do not recur to nitrogen-15 or carbon-13 isotope enrichment.

Sep 17, 2019

[NMR] solid-state NMR postdoc position available



Dear NMR community;

A postdoctoral position is available in my laboratory at UC Irvine (please see attached description.) The project involves developing and using multidimensional sequences for incorporating 2H into protein structure determination experiments in solids, using our unique quadruple-resonance MAS probe operating at 800 MHz. Anyone who is interested should email Rachel Martin (rwmartin@uci.edu). The formal application can be found on UCI’s website at https://recruit.ap.uci.edu/JPF05254. I will also be at the Alpine conference in Chamonix if any potential candidates who are attending the meeting would like to discuss the project with me.

Best,

Rachel Martin
DESCRIPTION


School of Physical Sciences
Department of Chemistry
Position: Postdoctoral Position – Solid-State NMR

A postdoctoral position is available at the University of California, Irvine in the area of protein structure determination by solid-state NMR, specifically of the aggregates of eye lens proteins found in cataract disease. The goal of this NIH-funded project is to develop and use advanced solid-state NMR methods for the study of complicated protein aggregates. The group has access to an 800 MHz instrument, equipped with solution-state and MAS probes, including a unique crossed-coil 1H/13C/2H/15N MAS probe purpose-built for these experiments. We also have two dedicated 500 MHz NMR instruments (one solids and one liquids), as well as a fully-equipped molecular biology
laboratory for sample preparation. The ideal candidate will be experienced in protein structure determination by MAS and interested in using novel instrumentation to solve biological problems.

The project supports solid-state NMR methods development and structure determination of wild-type human γS-crystallin in the transparent hydrogel state found in the healthy eye lens, as well as the aggregates formed by UV-light damaged proteins and cataract-related variants. The Martin group is experienced in preparation of crystallin proteins: isotopically labeled samples of the transparent hydrogel of the native crystallin and the aggregates associated with cataract have been prepared. As part of this project, new NMR methodology will be developed to investigate the structural factors related to γS-crystallin stability and solubility. Differential isotope labeling of peptide binders and variant crystallins will be used to identify specific residues involved in altered intermolecular interactions, followed by full structure determination of cataract aggregates. Extensive use will be made of deuterated samples and deuterium NMR in the context of multidimensional NMR experiments.

Candidates must have (or be about to earn) a Ph.D. in Chemistry, Physics, or a related discipline, and have experience solving protein structures using MAS NMR. Previous experience with pulse sequence development is desirable but not required.

Please apply online at https://recruit.ap.uci.edu/apply/JPF05254 with a cover letter that also describes your immediate and long-term research goals, a curriculum vitae including publications list, and names for three letters of reference (please do not solicit letters). Review of applications will begin on Oct 1, 2019 and will continue until the position is filled.

The University of California, Irvine is an Equal Opportunity/Affirmative Action Employer advancing inclusive excellence. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected categories covered by the UC nondiscrimination policy.

Rachel W. Martin
Professor of Chemistry
and Molecular Biology & Biochemistry
University of California, Irvine

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Sep 16, 2019

Hyperpolarized MR – What’s up Doc? #DNPNMR

Ardenkjaer-Larsen, Jan H. “Hyperpolarized MR – What’s up Doc?” Journal of Magnetic Resonance 306 (September 2019): 124–27.


Hyperpolarized MR by dissolution Dynamic Nuclear Polarization (dDNP) appeared on the scene in 2003. Since then, it has been translated to the clinic and several sites are now conducting human studies. This has happened at record pace despite all its complexities. The method has reached a pivotal point, and the coming years will be critical in realizing its full potential. Though the field has been characterized by strong collaboration between academia, government and industry, the key message of this perspective paper is that accelerated consensus building is of the essence in fulfilling the original vision for the method and ensuring widespread adoption. The challenge is to gain acceptance among clinicians based on strong indications and clear evidence. The future appears bright; initial clinical data looks promising and the scope for improvement is significant.

Sep 15, 2019

[NMR] Postdoctoral position in DNP-enhanced Magnetic Resonance #DNPNMR

Postdoctoral Researcher in DNP-enhanced Magnetic Resonance 

Barcelona, Spain

A postdoctoral research position is available starting from January 1st 2020 at the Institute for Bioengineering of Catalonia (IBEC), funded by an EU Horizon 2020 Future Emerging Technologies (H2020 FET-OPEN) grant. 

The position is fully funded for a period of up to 36 months.

The project we offer concerns real-time study of metabolism in engineered “organ-on-a-chip” tissue systems via hyperpolarized magnetic resonance. As part of our efforts to create non-invasive platforms for tailored drug testing, the successful candidate will be involved in the development of Carbon-13 hyperpolarization methods, as well as carrying out innovative work on the tissue engineering side of the project. 

Applicants should have a Ph.D. in engineering, physics, or chemistry. Previous experience in nuclear magnetic resonance or magnetic resonance imaging is necessary. Experience with 13C hyperpolarization, magnetic resonance hardware, data acquisition and signal/image processing is strongly favored. Knowledge of C++, Matlab, Python, and magnetic resonance spectrometer programming languages, will also be evaluated. Applicants should also have a strong track record of publications in peer-reviewed scientific journals and excellent communication skills.

Environment:
The Institute for Bioengineering of Catalonia (IBEC) is a research institute covering most bioengineering fields, from basic research to medical applications. IBEC is located at the Barcelona Science Park (PCB, www.pcb.ub.edu) in central Barcelona, and has strong connections with the nearby Bellvitge University Hospital (BUH). The researcher will have access to a commercial DNP instrument (3.35 T), and NMR spectrometers (60 MHz, 400 MHz, and 500 MHz 1H frequencies) in addition to clean room facilities, bio-laboratory space and computing equipment.

IBEC is also part of the Barcelona Institute of Science and Technology (BIST), a scientific foundation of seven of Catalonia’s research centres of excellence (CRG, IBEC, ICFO, ICIQ, ICN2, IFAE, IRB). The mission of BIST is to build new scientific collaborations among these centres, thus giving impulse to multidisciplinary projects to push ever further the frontiers of knowledge. Each BIST centre has reached a high level of excellence in its respective areas of expertise.

Contact: 
For informal inquiries about the position, please contact Dr. Irene Marco-Rius (imarco@ibecbarcelona.eu), Junior Leader fellow, Ramon laboratory, IBEC. 

Interested applicants should send the following materials: (1) a cover letter detailing research interests, (2) a curriculum vitae, (3) letters of recommendation from two referees.

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[NMR] PhD studentship in hyperpolarized 13C NMR #DNPNMR

PhD studentship in hyperpolarized 13C NMR
Barcelona, Spain

A PhD studentship is available starting from January 1st 2020 at the Institute for Bioengineering of Catalonia (IBEC), funded by an EU Horizon 2020 Future Emerging Technologies (H2020 FET-OPEN) grant. 

The project we offer concerns real-time study of metabolism in engineered “organ-on-a-chip” tissue systems via hyperpolarized magnetic resonance. As part of our efforts to create non-invasive platforms for tailored drug testing, the successful candidate will be involved in the development of Carbon-13 hyperpolarization methods, as well as carrying out innovative work on the tissue engineering side of the project. 

This multidisciplinary project is at the interface between physics, chemistry, biochemistry and tissue engineering.

Applicants should have a B.Sc./M.Sc. (or equivalent degree) in engineering, physics, or chemistry. Previous experience in 13C hyperpolarization, magnetic resonance hardware, data acquisition and signal/image processing areas of nuclear magnetic resonance is strongly favored. A knowledge of C++, Matlab, Python, and magnetic resonance spectrometer programming languages, will also be evaluated. Applicants should demonstrate excellent communication skills.

Environment:
The Institute for Bioengineering of Catalonia (IBEC) is a research institute covering most bioengineering fields, from basic research to medical applications. IBEC is located at the Barcelona Science Park (PCB, www.pcb.ub.edu) in central Barcelona, and has strong connections with the nearby Bellvitge University Hospital (BUH). The researcher will have access to a commercial DNP instrument (3.35 T), and NMR spectrometers (60 MHz, 400 MHz, and 500 MHz 1H frequencies) in addition to clean room facilities, bio-laboratory space and computing equipment.

IBEC is also part of the Barcelona Institute of Science and Technology (BIST), a scientific foundation of seven of Catalonia’s research centres of excellence (CRG, IBEC, ICFO, ICIQ, ICN2, IFAE, IRB). The mission of BIST is to build new scientific collaborations among these centres, thus giving impulse to multidisciplinary projects to push ever further the frontiers of knowledge. Each BIST centre has reached a high level of excellence in its respective areas of expertise.

Contact: 
For informal inquiries about the position, please contact Dr. Irene Marco-Rius (imarco@ibecbarcelona.eu), Junior Leader fellow, Ramon laboratory, IBEC. 

Interested applicants should send the following materials: (1) a cover letter detailing their research interests, (2) a curriculum vitae, (3) contact information for two referees.

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