Wednesday, September 13, 2017

Single-scan 13C diffusion-ordered NMR spectroscopy of DNP-hyperpolarised substrates #DNPNMR


Guduff, L., et al., Single-scan 13C diffusion-ordered NMR spectroscopy of DNP-hyperpolarised substrates. Chemistry, 2017: p. n/a-n/a.


Diffusion-ordered NMR spectroscopy (DOSY) is a powerful approach for the analysis of molecular mixtures, yet its application range is limited by the relatively low sensitivity of NMR. We show here that spectrally resolved 13C DOSY data can be collected, in a single scan, for substrates hyperpolarised by dissolution dynamic nuclear polarisation (D-DNP), which provides signal enhancements of several orders of magnitude. For this we use a convection-compensation pulse scheme, which we also analyse by numerical simulation. The proposed method further allows the acquisition of several consecutive DOSY spectra in a single D-DNP experiment.