Wednesday, June 28, 2017

Dynamic Nuclear Polarization of beta-Cyclodextrin Macromolecules #DNPNMR


Caracciolo, F., et al., Dynamic Nuclear Polarization of beta-Cyclodextrin Macromolecules. J Phys Chem B, 2017. 121(12): p. 2584-2593.


1H dynamic nuclear polarization and nuclear spin-lattice relaxation rates have been studied in amorphous complexes of beta-cyclodextrins doped with different concentrations of the TEMPO radical. Nuclear polarization increased up to 10% in the optimal case, with a behavior of the buildup rate (1/TPOL) and of the nuclear spin-lattice relaxation rate (1/T1n) consistent with a thermal mixing regime. The temperature dependence of 1/T1n and its increase with the radical concentration indicate a relaxation process arising from the modulation of the electron-nucleus coupling by the glassy dynamics. The high-temperature relaxation is driven by molecular motions, and 1/T1n was studied at room temperature in liquid solutions for dilution levels close to the ones typically used for in vivo studies.