From the Ampere Magnetic Resonance List
I have a Postdoctoral Associate position available immediately to study functional amyloid formation by Streptococcus mutans, particularly as it relates to biofilm development. The focus of this project is to characterize the ultrastructure and amyloid properties of the adhesion P1 and other amyloidogenic surface proteins utilizing solution NMR, DNP-enhanced solid state NMR, cryoelectron microscopy, and diffraction techniques to study their structure in fibrillar and nonfibrillar forms. This project is in collaboration with Dr. Jeannine Brady, an expert in bacterial adhesion and biofilm formation.
NMR facilities include several solid state and solution spectrometers located at UF and the National High Magnetic Field Laboratory ranging from 500-900 MHz, including a 600 MHz ssNMR instrument with DNP capabilities. Extensive facilities for protein production and purification, cryoelectron microscopy, x-ray crystallography, and high performance computing are in close proximity to the Long and Brady labs as part of the UF centers for structural biology and high performance computing.
A Ph.D. and English language proficiency are required. Applicants should have a strong background in biomolecular NMR spectroscopy (either solution or solid state), molecular microbiology, and biochemistry with an emphasis on protein structure/dynamics/function. Experience with standard cloning and recombinant DNA methodologies, protein chemistry, chromatography, and heterologous protein expression and purification is necessary.
Familiarity with techniques to evaluate protein-protein interactions, protein crystallization and X-ray diffraction methodologies, mass spectrometry, and electron microscopy will be given preference. Also desirable would be familiarity with assays to assess amyloid fibrillization including use of fluorescent dyes and spectral shift assays, Congo-red induced birefringence, confocal microscopy and immunogold electron microscopy.
This is a grant-funded position and is annually renewable based on the availability of research funds. Salary commensurate with NIH postdoctoral stipend guidelines.
For information on applying and any specific question related to the position, please contact me at firstname.lastname@example.org.
Joanna R. Long, PhD
Associate Professor of Biochemistry & Molecular Biology
Director, Advanced Magnetic Resonance Imaging & Spectroscopy Facility
Assoc. Director, National High Magnetic Field Laboratory
McKnight Brain Institute, LG-187
University of Florida
Gainesville, FL 32605
(352)294-8399 ***NOTE: phone number changed 8/22/2016***
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