Monday, August 3, 2015

Solid-state NMR methods for oriented membrane proteins


This review article gives a comprehensive overview of the field of solid-state NMR spectroscopy on oriented samples. These experiments are typically less demanding on instrumentation since experiments are performed on static samples, without the need of spinning. While this article is not directly related to DNP-NMR spectroscopy, DNP-NMR on static samples will certainly become more interesting in the future.


Hansen, S.K., et al., Solid-state NMR methods for oriented membrane proteins. Prog. NMR. Spec., 2015. 88-89: p. 48-85.


Oriented-sample solid-state NMR represents one of few experimental methods capable of characterising the membrane-bound conformation of proteins in the cell membrane. Since the technique was developed 25 years ago, the technique has been applied to study the structure of helix bundle membrane proteins and antimicrobial peptides, characterise protein-lipid interactions, and derive information on dynamics of the membrane anchoring of membrane proteins. We will review the major developments in various aspects of oriented-sample solid-state NMR, including sample-preparation methods, pulse sequences, theory required to interpret the experiments, perspectives for and guidelines to new experiments, and a number of representative applications.