Wednesday, January 7, 2015

Using [1- C]lactic acid for hyperpolarized C MR cardiac studies


Chen, A.P., et al., Using [1- C]lactic acid for hyperpolarized C MR cardiac studies. Magn Reson Med, 2014: p. n/a-n/a.


PURPOSE: Hyperpolarized [1-13 C]lactate in solution may be a clinically relevant and safe substrate for real time MR investigations of key metabolic pathways. The potential of using hyperpolarized [1-13 C]lactate for magnetic resonance studies of cardiac metabolism in vivo was explored. METHODS: Neat [1-13 C]lactic acid was hyperpolarized using the dynamic nuclear polarization process. Cardiac MR spectroscopy experiments were performed in vivo using hyperpolarized [1-13 C]lactate and [1-13 C]pyruvate in solutions. RESULTS: A high degree of polarization was achieved for [1-13 C]lactate in solution (16.7%). 13 C-bicarbonate was observed in rat hearts in vivo after either hyperpolarized [1-13 C]lactate or hyperpolarized [1-13 C]pyruvate was infused, but lower 13 C-bicarbonate to substrate ratio was observed with hyperpolarized [1-13 C]lactate infusions. The response of 13 C-bicarbonate signal as a function of hyperpolarized [1-13 C]lactate doses was also investigated and a saturation of 13 C-bicarbonate signal was observed at the highest dose of [1-13 C]lactate used (0.69 mmol/kg). CONCLUSION: This study demonstrated that the use of neat [1-13 C]lactic acid as the DNP sample is a potential alternative to [1-13 C]pyruvic acid for cardiac hyperpolarized 13 C MR studies. Hyperpolarized [1-13 C]lactate may enable noninvasive assessment of cardiac PDH flux in cardiac patients in the near future. Magn Reson Med, 2014. (c) 2014 Wiley Periodicals, Inc.