Monday, January 5, 2015

Let's start the new year with an article that is already a bit older. However, I just came across it recently and I don't think I posted it already. It is a very nice overview about Dissolution DNP by Walther Koeckenberger.

Köckenberger, W., Dissolution Dynamic Nuclear Polarization, in eMagRes. 2007, John Wiley & Sons, Ltd.

Dissolution DNP (dissDNP) is an experimental strategy that can be used to substantially increase the nuclear spin polarization in liquid-state samples. The strategy is based on DNP carried out at cryogenic temperatures with a subsequent fast temperature rise of the sample that is achieved by dissolving the initially frozen sample in a hot solvent. The strategy has opened up novel avenues in medical diagnostic imaging, as 13C-labeled compounds can first be prepared with high-spin polarization by dissDNP before their administration to humans or animals, followed by their in vivo detection using MRI. The use of fast spectroscopic imaging acquisition schemes makes it possible to observe the in vivo spatial distribution of 13C-labeled compounds and monitor their in vivo metabolic turnover due to the high-spin polarization and the related increase in sensitivity. The strategy has also shown to be useful in measuring in vitro metabolism in cell cultures with high time resolution, and in enabling novel experimental protocols for in vitro molecular dynamics studies.