Friday, April 11, 2014

Dynamic Nuclear Polarization NMR Spectroscopy: Revealing Multiple Conformations in Lipid-Anchored Peptide Vaccines


Koers, E.J., et al., Dynamic Nuclear Polarization NMR Spectroscopy: Revealing Multiple Conformations in Lipid-Anchored Peptide Vaccines. Angew Chem Int Ed Engl, 2013. 52(41): p. 10905-10908.


Sensitivity is the key: Dynamic nuclear polarization NMR spectroscopy provides structural information on liposomal vaccines targeting Alzheimer's disease. DMPC/DMPG/Cholesterol mainly stabilizes extended structures of the lipid-anchored peptide, while in DMTAP/Cholesterol liposomes the peptide adopts a multitude of conformations including random-coil and extended structures.